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Abstract
Pharmacologically active xanthone compounds isolated from Swertia przewalskii pissjauk were well separated by capillary electrophoresis (CE) within 5 min, using a running buffer of 25 mM disodium tetraborate at pH 9.0. Quantitative determination was shown to be possible because regression equations revealed a linear relationship between the peak area of each constituent and its concentration, with correlation coefficients of 0.9972–0.9994. The relative standard deviations were between 0.44%–0.73% for migration times and 2.52%–4.28% for peak areas. The dissociation constant of 1,7‐O‐β‐D‐glucopyranosyl‐8‐hydroxy‐3,7‐dimethoxyxanthone, 1,8‐dihydroxy‐3, 7‐dimethoxy‐xanthone and 1,7‐di‐hydroxy‐3,8‐dimethoxyxanthone were also measured by the CE method, giving a value of 9.04, 8.94, and 8.59, respectively.
Acknowledgments
This work was financially supported by National Sciences Foundation of China (No. 29825112) and The Chinese Academy of Sciences (No. KJ951‐A1‐507).