Separation of High Purity Przewaquinone A by High‐Speed Countercurrent Chromatography

Abstract

High‐speed countercurrent chromatography (HSCCC) was used to purify przewaquinone A from the extract of Salvia miltiorrhiza Bunge using a two‐phase solvent system composed of carbon tetrachloride–methanol–water–n‐hexane, at an optimum volume ratio of 3:3:2:1. The method yielded 15.3 mg of 98% przewaquinone A from 100 mg of a crude sample containing przewaquinone A, at 16.0% in a single run. The recovery of przewaquinone A is as high as 93.7%. Identification was performed by IR, UV, ¹H NMR, ¹³C NMR, and FAB‐MS.

Keywords:

• Countercurrent chromatography
• Salvia miltiorrhiza
• Przewaquinone A