Abstract
To be able to design a J‐type centrifuge for a given need, a method of being able to predict peak elution is required. Predicting peak elution will also allow the user to optimise the process parameters for his or her needs. Such predictions require an accurate knowledge of the volume of the stationary phase retained in the coil for a given set of operating conditions. This paper builds upon an experimental relationship in that the stationary phase retention decreases proportionally to the square root of the mobile phase flow rate. Combining this experimental relationship with the hypothesis that the pressure drop across a coil is independent of mobile phase flow rate, and assuming that the mobile phase flow is laminar, the equation below is derived:
Notes
aAn accuracy of 3 mL is used as it allows for small inaccuracies caused by evaporation, spillages, and wetting of surfaces above the meniscus in measuring cylinders.
bThe stationary phase is displaced from a coil by the mobile phase; therefore the volume of mobile phase in a coil (V m ) equals the volume of stationary phase displaced from a coil.