Separation of Dammarane‐Saponins from Notoginseng, Root of Panax notoginseng (Burk.) F. H. Chen, by HSCCC Coupled with Evaporative Light Scattering Detector

Abstract

High‐speed countercurrent chromatography (HSCCC) coupled with evaporative light scattering detection, was applied to the separation of saponins from notoginseng, the main root of Panax notoginseng (Burk.) F. H. Chen. Five individual dammarane saponins have been isolated with the solvent systems composed of CHCl₃/MeOH/2‐BuOH/H₂O (5:6:1:4, v/v/v/v) and EtOAc/1‐BuOH/H₂O (1:1:2, v/v/v), successively. They were identified as ginsenoside‐Rg₁, ginsenoside‐Rd, notoginsenoside‐R₁, ginsenoside‐Re, and ginsenoside‐Rb₁ by FAB‐MS and ¹³C NMR, together with HPLC and TLC analysis.

Keywords:

• CCC
• Evaporative light scattering detector
• Saponins
• Ginsenosides