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Abstract
Analytical high‐speed countercurrent chromatography (HSCCC) was used for the systematic selection and optimization of the two‐phase solvent system to separate lutein from the crude extract of the marigold flower petal. Lutein was obtained by preparative HSCCC with a two‐phase solvent system composed of n‐heptane–chloroform–acetonitrile (10:3:7, v/v/v). HPLC analyses of the lutein revealed that it was over 98.5% purity. The final identification was performed by UV–VIS absorption, 1H‐NMR, and 13C‐NMR Spectra.
Acknowledgment
Financial support from Beijing Commission of Science & Technology is gratefully acknowledged.