Abstract
The primary glycosides in the hybrid Digitalis ambigua × Digitalis lanata leaves were determined by HPLC and compared with the measurement of lanatosides in D. ambigua and D. lanata leaves. The extract of dry leaf powder with chloroform:ethanol (1:2, v/v) was submitted to Sep‐Pak cartridges prior to HPLC analysis. HPLC was performed on a TSK‐GEL AMIDE‐80 column using acetonitril:water (96:4). The effluent was monitored by ultraviolet (UV) absorption at 220 nm. The quantitation was carried out by the internal standard method. The amount of lanatoside C per 100 mg of dry leaf powder samples of the hybrid D. ambigua × D. lanata and D. lanata were 227.3 µg and 134.4 µg, respectively. The amount of lanatoside C in the hybrid D. ambigua × D. lanata was higher than in D. lanata.
Acknowledgment
The authors thank Mr. Masaru Yamamoto of the Medicinal Plant Garden of Hokuriku University for providing the leaves of Digitalis used in this study.