Analysis of Stibonic Acids by Ion Exchange Chromatography with ESI‐MS/Photodiode Array Detection

Abstract

A method utilizing the counter anion exchange properties of aqueous ammonium acetate at pH 9, increasing in concentration linearly from 0 to 0.1 M NH₄OAc, using a Hamilton PRP‐X100 anion exchange column, is presented for the resolution of aromatic stibonic acids and their detection by UV and ESI mass spectrometry. Additional phase‐bonded silica or polymer backed C₈ and C₁₈ column types, eluted with various counter ion solutions (KClO₄, NH₄COOH, NaOH, NaH₂PO₄) were evaluated for suitability for stibonic acid analysis.

^#The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organization imply endorsement by the U.S. Government. This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. N01‐CO‐12400. Accordingly, the U.S. Government retains a non‐exclusive, royalty‐free license to publish or reproduce the published form of this contribution, or allow others to do so, for U.S. Government purposes.

Keywords:

• Stibonic acids
• Ion exchange chromatography
• ESI‐MS
• Photodiode array detection

Acknowledgments

Notes

^#The content of this publication does not necessarily reflect the views or policies of the Department of Health and Human Services, nor does mention of trade names, commercial products, or organization imply endorsement by the U.S. Government. This project has been funded in whole or in part with Federal funds from the National Cancer Institute, National Institutes of Health, under Contract No. N01‐CO‐12400. Accordingly, the U.S. Government retains a non‐exclusive, royalty‐free license to publish or reproduce the published form of this contribution, or allow others to do so, for U.S. Government purposes.