Column Switching Technique, Assisted On‐Line Matrix Elimination, and Chiral Analysis of R‐ and S‐1‐Phenyl‐1‐butanol in Biphasic Saccharomyces cerevisiae Mediated Culture

Abstract

The matrix interference subjected by R‐ and S‐1‐phenyl‐1‐butanol in the biphasic Saccharomyces cerevisiae CCRC 21443 mediated culture cannot be eliminated by chiral high‐performance liquid chromatography (HPLC) with a single column of either Chiralcel OB or Chiralpak AD. The use of a single column also results in a long analysis time. The application of the column switching technique can hyphenate the Chiralcel OB column and the Chiralpak AD column together, to resolve not only the matrix interference, but a reduction of the analysis time for these chiral products in a complex medium. This coupled column system has an accuracy greater than 98.6% and a high precision of less than 1.6% relative standard deviation (RSD). The developed technique for on‐line sample clean‐up and analysis of phenyl‐n‐propyl ketone, R‐ and S‐1‐phenyl‐1‐butanol in the culture is successful.

Keywords:

• Column switching
• Matrix elimination
• Chiral
• Saccharomyces cerevisiae

Acknowledgment

The authors gratefully thank the National Science Council, under grant No. NSC 89‐2113‐M‐033‐016, for their financial support.