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Abstract
A rapid and simple method for determination of cefaclor in human plasma samples, by high‐performance liquid chromatography (HPLC), was developed. This method includes a deproteinization with perchloric acid as extraction procedure. Plasma extracts were analyzed on a reversed phase column eluted with a mixture of acetonitrile and 0.01 M sodium dihydrogen phosphate solution, adjusted to pH 6.5 with potassium hydroxide (7:93, v/v), and detected by absorbance at 260 nm. Retention times for cefaclor and the internal standard were 5.2 and 7.2 min, respectively. The method was linear in the range of 0.5–30 µg/mL and the detection limit of the method was 100 ng/mL. This method was useful and suitable for determination of cefaclor after oral administration in healthy volunteers, and could be used for bioavailability and bioequivalence studies of the drug.
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Acknowledgment
The authors greatly appreciate the bibliographic assistance of Hector Vázquez. V. Granados‐Soto is a recipient of a sabbatical fellowship from CONACYT.
