Peak Purity Determination with a Diode Array Detector

Abstract

Peak purity analysis is an evaluation technique for detecting the presence of coeluting impurities in high performance liquid chromatography (HPLC) data. Running a peak purity check prior to analytical quantitation helps to ensure accuracy. In the development of analytical methods, peak purity analysis can reveal the presence of contamination during standardization and, by doing so, prevent the subsequent generation of false analytical data. Peak purity analysis is also a useful addition to routine quality control procedures, especially in the analysis of pharmaceuticals and food products, for which contamination and quality of results are critical.

^#Reprinted from the Encyclopedia of Chromatography (© 2003); URL: http://www.dekker.com/servlet/product/productid/E‐ECHR.

Keywords:

• Diode array detector
• Peak purity
• Signal overlay
• Spectrum normalization

Notes

^#Reprinted from the Encyclopedia of Chromatography (© 2003); URL: http://www.dekker.com/servlet/product/productid/E‐ECHR.