Separation of Peptides and Proteins by Capillary Electrochromatography

Abstract

Capillary electrochromatography (CEC) is a separation method that has gained increasing attention in recent years. It combines the advantages of high performance liquid chromatography (HPLC) selectivity with the high efficiency of electrophoresis. Due to its promise of high peak capacities, CEC appears to be promising for resolution of complex mixtures, such as those generated during protein and peptide mapping. However, direct transfer of HPLC and capillary zone electrophoresis (CZE) methods to CEC is not trivial due to the unique requirements for CEC in terms of stationary phase and operating conditions. In this paper, we discuss the various approaches undertaken for the CEC separation of peptides and proteins.

Keywords:

• Peptides and proteins
• Capillary electrochromatography
• Time‐of‐flight
• Mass spectrometry

Acknowledgments

The authors would like to thank Merck & Co., Inc. for the support of our work.