Abstract
The method for the determination of acarbose in human plasma is described, using high‐performance liquid chromatographic separation with tandem mass spectrometric detection. Samples were prepared using solid phase extraction and separated on a Zorbax SB C18 column with a mobile phase consisting of water, acetonitrile, and triflouroacetic acid. Detection was performed by a TSQ quantum mass spectrometer in the selected reaction monitoring (SRM) mode using electrospray ionization (ESI). The method has a chromatographic elution time of 3 min and was linear within the range of 100–1000 ng/mL. The intra‐ and inter‐run accuracy and precision, calculated from quality control (QC) samples, was less than 11%.
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Acknowledgments
The authors are thankful to Wockhardt Research Centre, Aurangabad and Head‐Department of Chemical Technology, Dr Babasaheb Ambedkar Marathwada University, Aurangabad, for providing the facilities for this research work.