Retention Process in Normal‐Phase TLC Systems

Abstract

The influence of mobile phase composition on the retention of selected test analytes in different normal‐phase TLC systems has been studied. A novel adsorption model for an accurate prediction of the analyte retention in the thin‐layer chromatography with binary mobile phase has been proposed. The performance of the model was compared with the retention models reported in the literature. All the models were verified for different TLC systems by three criteria: the sum of squared differences between the experimental and theoretical data, the approximation of the standard deviation, and the Fisher test.

Keywords:

• Mobile phase composition
• Polar bonded stationary phases
• Retardation factor
• Retention models
• TLC