Identification and Quantification of Bis‐GMA and Teg‐DMA Released from Dental Materials by HPLC

Abstract

Most of the composites and sealants used in dentistry are based on bisphenol A diglycidylether methacrylate (Bis‐GMA) and triethylenglycol dimethacrylate (Teg‐DMA). Reports revealed that in situ polymerization is not complete and that free monomers can be detected by different analytical methods. We studied residual Bis‐GMA and Teg‐DMA after in vitro polymerization by high performance liquid chromatography (HPLC) after 24 hr, 7, and 14 days. For quantitative analysis, an RP column (250 × 4.6 mm; 5 µm) as stationary phase and isocratic elution with CH₃CN:H₂O (60:40) was used. A calibration curve was constructed using standard solutions of Bis‐GMA and Teg‐DMA in the concentration range from 1 × 10⁻³ to 5 × 10⁻⁵ mol/L. The limits of detection (LOD) and quantification (LOQ) for both analytes were determined (for Bis‐GMA: 0.0076 and 0.0229 mmol/L; and for Teg‐DMA: 0.0123 and 0.0373 mmol/L, respectively). The accuracy of the method was confirmed by standard additions. The HPLC analysis of residual components revealed that Bis‐GMA was released from commercial samples in the highest concentration after 14 days. Teg‐DMA was detected only in one of all analyzed dental materials.

Keywords:

• Bis‐GMA
• TEG‐DMA
• Identification
• Dental materials
• HPLC