Lipophilicity of Selected Bile Acids as Determined by TLC. II. Investigations on RP18W Stationary Phase

Abstract

The bile acids, such as: cholic (C), glycocholic (GC), glycodeoxycholic (GDC), chenodeoxycholic (CDC), deoxycholic (DC), lithocholic (LC), and glycolithocholic (GLC) acid were investigated with the use of reversed phase thin‐layer chromatography on RP18W (E. Merck, #1.14296), using methanol–water, organic mixture (acetonitrile–methanol 50:50, v/v)–water, acetone–water, dioxane–water in different volume compositions as a mobile phase. The chromatographic parameters of lipophilicity (R _MW and ϕ₀) of studied bile acids were determined. Lipophilic parameters (R _MW and ϕ₀) were compared both, with measured (log P _exp), and calculated partition coefficients (A log P _S, IA log P, log P _KOWIN, x log P, c log P, log P _Rekker). The most significant correlation was found between lipophilic parameters R _MW and ϕ₀ and log P _KOWIN values. The results indicate that chromatographic R _M values may be used as a measure of lipophilicity of the investigated bile acids.

Keywords:

• Bile acids
• RP‐HPTLC
• RP18W
• Lipophilicity