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Abstract
A precise and accurate flow injection analysis (FIA) method for the determination of mitomycin‐C (MMC) in pharmaceutical flacons is described. Britton–Robinson (BR) buffer at pH 7.0 was used as a carrier solvent at a flow‐rate of 1.0 mL min−1. MMC signals were detected at 363 nm using a UV‐spectrophotometric detector. The intra‐ and inter‐assay precisions were less than 2.0%. The calibration curves were also linear in the concentration range of 2.0 × 10−6–1.0 × 10−5 M for intra‐ and inter‐day studies with high correlation coefficients. The limit of detection (LOD) and limit of qua.gification (LOQ) values were found to be 1.93 × 10−8 and 6.45 × 10−8 M, respectively. The results obtained with the application of the method were compared with those of high performance liquid chromatography (HPLC) and UV‐spectrophotometry. It was found that there was insignificant difference between the methods.
Acknowledgment
The authors appreciate Anadolu University, Council of Research Project Fund for their support of chemicals and instruments.