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Abstract
The optimisation of the selectivity of two columns coupled in series has been investigated in HPLC. Two columns with different polarities (PEG and CN) were coupled in series via a T connector and the retention of analytes chromatographed on the column system was tuned by varying the individual mobile phase flows in the coupled columns. The flow rate ratio necessary for receiving an optimum selectivity was calculated on the basis of the measured retention factors on the individual columns. The performance of this method has been studied by the separation of a model mixture of 17 analytes with various functionalities.