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Abstract
The matrix oligo‐β‐cyclodextrin‐Sepharose HP was synthesized and successfully applied in the separation of puerarin from pueraria flavones. The optimal conditions of preparation were obtained. The separation was carried out with 7% acetic acid solution (V %) at a flow rate of 10 mL/min, after 300 mg flavones in 20 mL 5% ethanol (V %) were injected in a preparative column (350 mm×44 mm i.d.). The puerarin fraction was collected and further purification was performed. The purity of puerarin obtained was higher than 97%. The recovery of the whole procedure reached 32.4%.
Acknowledgments
The authors thank for the financial supports of the National Natural Science Foundation of China (29976004, 20136020, 50373003, and 20325622), Beijing Natural Science Foundation (2032013), Specialized Research Fund for the Doctoral Program of Higher Education (20030010004), as well as Teaching and Research Award Program for Outstanding Young Teacher in Higher Education Institute.
