Preparation of Triterpene Saponins from the Fruit of Momordica Charantia L. by High Speed Countercurrent Chromatography (HSCCC)

Abstract

High speed countercurrent chromatography (HSCCC) was used for the separation of triterpene saponins of two components from silica gel column chromatography of bitter gourd crude extract (Momordica charantia L), with two biphasic solvent systems composed of methyl tert‐butyl ether‐n‐butanol‐methnaol‐water in the proportions of 1∶2∶1∶5 and 1∶3∶1∶5 (v/v), respectively. Four compounds, goyaglycoside‐e, momordicoside L, goyaglycoside‐a, and momordicoside K were obtained and confirmed by means of ESI‐MS, ¹H‐ and ¹³C‐NMR.

Keywords:

• Momordica charantia
• Triterpene saponins
• High speed countercurrent chromatography
• Preparative isolation

Acknowledgment

Authors are grateful for the funding from Sino‐Germany Research Foundation, Item No. GZ051‐4 (148).