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Abstract
The method uses preparative centrifugal precipitation chromatography comprising a seal‐free continuous‐flow centrifuge machine equipped with a plastic disk and a spiral groove which accommodates convoluted tubing. A dialysis membrane is inserted into the convoluted tubing to separate proteins according to their solubility in ammonium sulfate solution. The system relies on the original model of centrifugal precipitation chromatography, which employs the centrifugal force and a concentration gradient of precipitants to perform a repetitive process of precipitation and dissolution of protein molecules. Compared to the previous model, the current model results in a higher yield of separated compounds.