Abstract
We employ NMR structure determination, thermodynamics, and enzymatics to uncover the structural, thermodynamic and enzymatic properties of α/β-ODNs containing 3′-3′ and 5′-5′ linkages. RNase H studies show that α/β-gapmers that are designed to target erbB-2 efficiently elicit RNase H activity. NMR structures of DNA · DNA and DNA · RNA duplexes reveal that single α-anomeric residues fit well into either duplex, but alter the dynamic properties of the backbone and deoxyriboses as well as the topology of the minor groove in the DNA · RNA hybrid.
Acknowledgments
Notes
*Please see color insert for Figure 4.