SYNTHESIS OF OLIGONUCLEOSIDE BORANOPHOSPHATES VIA AN H-PHOSPHONATE METHOD WITHOUT NUCLEOBASE PROTECTION

Abstract

Short oligonucleoside boranophosphates containing all four nucleosides were synthesized on solid support using base-unprotected nucleoside H-phosphonate monomers. This strategy avoided irreversible base modifications during the boronation procedure. Structures of the boranophosphate oligomers were confirmed by ¹H, ³¹P, ¹⁰B NMR and MS analysis as well as by enzymatic hydrolysis.

ACKNOWLEDGMENT

This work was supported by grant NIH 5R01-GM57693 to B.R.S.