2′-O-MODIFIED OLIGORIBONUCLEOTIDES WITH TERMINAL 3′-3′-INTERNUCLEOTIDE LINKAGE AND THEIR DERIVATIVES

Abstract

A high RNA binding affinity and nuclease resistance of 2′-O-modified (2′-O-methyl, 2′-O-tetrahydropyranyl) oligoribonucleotides containing the “inverted” T at the 3′-end have been shown. The synthesis and properties of new photoactivatable perfluoroarylazide derivatives of these oligoribonucleotides are discussed.

ACKNOWLEDGMENTS

This work was supported, in part, by RFBR grant N 99-04-49537, grant from Russian State Program “Gene targeted biologically active compounds as antiviral and anticancer drugs” and by Award_REC-008 of CRDF. We are grateful to Dr. Dmitrii Pyshnyi for his help with T_m measurements and to Mrs. Nina Komarova for skilful analysis. We thank Dr. Repkova M.N., Dr. Ivanova E.M. and Dr. Levina A.S. for their helpful discussion.