A NOVEL AFFINITY CHROMATOGRAPHY METHOD FOR THE CO-PURIFICATION OF DEOXYCYTIDINE KINASE AND CYTIDINE DEAMINASE

Hiroyuki Uchida Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 3-9-1 Bunkyo, Fukui, 910-8507, JapanCorrespondence[email protected]

Hidetaka Morinaga Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 3-9-1 Bunkyo, Fukui, 910-8507, Japan

Takashi Misaki Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 3-9-1 Bunkyo, Fukui, 910-8507, Japan

Takamichi Miyazaki Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 3-9-1 Bunkyo, Fukui, 910-8507, Japan

Takayuki Uwajima Department of Applied Chemistry and Biotechnology, Faculty of Engineering, Fukui University, 3-9-1 Bunkyo, Fukui, 910-8507, Japan

Tohru Obata Department of Experimental Therapeutics, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-0934, Japan

Yoshio Endo Department of Experimental Therapeutics, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-0934, Japan

Akira Matsuda Graduate School of Pharmaceutical Sciences, Hokkaido University, Kita 12, Nishi 6, Kita-ku, Sapporo, 060-0812, Japan

Takuma Sasaki Department of Experimental Therapeutics, Cancer Research Institute, Kanazawa University, 13-1 Takaramachi, Kanazawa, 920-0934, Japan

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Abstract

By affinity chromatography with Sepharose coupled to 2′-deoxy-1-β-D-ribofuranosyl-N ⁴-dodecanoylcytosine, deoxycytidine kinase and cytidine deaminase were purified 1,950- and 2,240-fold, respectively, from Ehrlich carcinoma cells, and their enzyme activities for several deoxycytidine analogs were investigated.

ACKNOWLEDGMENTS

This investigation was supported in part by a Grant-in-Aid for Scientific Research on Priority Areas from the Ministry of Education, Science, Sports, and Culture of Japan, and by the 2nd-Term Comprehensive Ten-Year Strategy for Cancer Control from the Ministry of Health and Welfare of Japan.

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A NOVEL AFFINITY CHROMATOGRAPHY METHOD FOR THE CO-PURIFICATION OF DEOXYCYTIDINE KINASE AND CYTIDINE DEAMINASE

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A NOVEL AFFINITY CHROMATOGRAPHY METHOD FOR THE CO-PURIFICATION OF DEOXYCYTIDINE KINASE AND CYTIDINE DEAMINASE

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