CHEMICAL SYNTHESIS OF OLIGONUCLEOTIDES CONTAINING (M + 4) GUANINE

Abstract

HPLC MS/MS has shown great potential in the measurement of DNA oxidative damage. Its accuracy depends on the use of multiply isotopically labelled internal standards. In this report, multiply isotopically labelled (M + 4) guanine internal standards were prepared in the form of base, nucleoside, as well as DNA oligomer. To our knowledge, this is the first chemical synthesis of oligomers containing (M + 4) guanine, and we believe that they can be used to develop a procedure that can make further improvement to the existing analytical procedures.

Keywords:

• DNA Oxidative Damage
• Isotopically Labelled Guanine
• Internal Standard
• Oligonucleotide