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Abstract
The effects of Fe limitation and bicarbonate addition to the nutrient medium on the organic acid metabolism were investigated in the root tips of various grapevine genotypes. Cuttings of two limestone‐tolerant and two limestone‐susceptible Vitis genotypes were grown for four weeks in nutrient solutions containing 10 or 0.5 µM Fe. The effect of bicarbonate addition (5 mM) was studied for two of these genotypes. Compared to 10 µM, Fe limitation (0.5 µM) significantly increased citrate concentration in root tips after 2 weeks, and malate concentration after 4 weeks. When Fe limitation and bicarbonate addition were combined, citrate and malate concentrations were significantly increased after 2 weeks. Fe limitation or addition of 5 mM bicarbonate had a larger effect on citrate than on malate concentrations. Addition of 5 mM bicarbonate discriminated more clearly tolerant and susceptible genotypes than Fe limitation. High malate and citrate concentrations in the roots were associated to high PEPC activities. These results confirm that root organic acid metabolism is involved in grapevine response to Fe deficiency stress. If verified on a larger range of genotypes, a procedure using bicarbonate effect on root tip citrate concentration could be proposed to screen limestone‐tolerant Vitis rootstocks.