SOLID PHASE OLIGONUCLEOTIDE SYNTHESIS BY USING β-CE H-PHOSPHONATE APPROACH

ABSTRACT

A new approach to the solid phase synthesis of oligonucleotide is described, which is based on oxidative coupling of nucleoside 3′-β-CE H-phosphonates in presence of N-Bromosuccinimide (NBS) as a coupling agent.

ACKNOWLEDGMENTS

We would like to thank The Department of Atomic Energy, Mumbai for financial assistance. Nikhil U. Mohe would like to thank The Department of Science and Technology, New Delhi, for the financial assistance. National facility of high field NMR, TIFR, Mumbai is gratefully acknowledged.