Cannabinoid CB1 Receptor Chemical Affinity Probes: Methods Suitable for Preparation of Isopropyl [11,12-³H]Dodecylfluorophosphonate and [11,12-³H]Dodecanesulfonyl Fluoride

Abstract

Preparations of isopropyl dodec-11-enylfluorophosphonate and dodec-11-enesulfonyl fluoride are described along with conditions for their reduction with hydrogen (or potentially tritium) gas to the 1-dodecane derivatives as candidate high-potency (IC₅₀ 0.5–7 nM) chemical affinity probes for the mouse brain cannabinoid CB1 receptor and fatty acid amide hydrolase.

Keywords:

• Cannabinoid CB1 receptor
• Chemical affinity probes
• Fatty acid amide hydrolase
• Dodecanesulfonyl fluoride
• Isopropyl decylfluorophosphonate

Acknowledgment

This work was supported by Grant R01 ES08762 from the National Institute of Environmental Health Sciences (NIEHS), NIH, and its contents are solely the responsibility of the authors and do not necessarily represent the official views of the NIEHS, NIH.