Synthesis and Characterization of the Copper(II) Complex with 2,2‐bis(1H,3H,5H)Pyrimidine‐4,6‐dione‐1,2‐diiminoethane: Fluorescence Quenching Studies in Proteins

Abstract

The new ligand 2,2‐bis(1H,3H,5H)pyrimidine‐4,6‐dione‐1,2‐diiminoethane (H₂SB) has been synthesized and characterized by elemental analyses, IR, NMR spectroscopy. Its copper complex has also been prepared and charaterized by various physico‐chemical methods. The reactivity of the 2,2‐bis(1H,3H,5H)pyrimidine‐4,6‐dione‐1,2‐diiminoethanecopper(II) complex towards protein has been studied spectrophotometrically. Affinity constants and binding capacity constants of protein with 2,2‐bis(1H,3H,5H)pyrimidine‐4,6‐dione‐1,2‐diiminoethanecopper(II) [Cu(SB)] (SB = doubly deprotonated Schiff base) have been calculated.

Acknowledgments

We are thankful to the Indian Institute of Technology, Delhi, and the Tata Institute of Fundamental Research, Mumbai, for spectral studies.