BINDING INTERACTION BETWEEN CHONDROITIN SULFATE AND METHYLENE BLUE BY SPECTROPHOTOMETRY

ABSTRACT

Chondroitin sulfate (CS) has a variety of biological activities, most of them due to chondroitin sulfate's sulfonic acid groups and carboxyl groups as well. To gain insight into the mechanism of interaction between the spectroscopic probe and chondroitin sulfate, we have used Methylene Blue (MB), one cationic dye, by a spectrophotometric method. This paper developed a new experimental method for determining the maximum binding number Ne, which expresses the binding ability of this dye with CS. Meanwhile, By using an interaction theory model we have established, the maximum binding number Nc can be calculated from the linear regression equation. The research results show that the Ne value is in agreement with that of Nc.

Keywords:

• Chondroitin sulfate
• Methylene Blue
• Maximum binding number
• Spectroscopic probe

Acknowledgments