Abstract
The current HPLC calibration standard for measuring alpha acids and beta acids in hops and hop products is International Calibration Extract Three (ICE-3), a deoiled CO2 hop extract. Laboratories that use ICE-3 must warm and mix the calibration extract to insure that it is homogenous prior to use. Alternatively, one can prepare crystalline dicyclohexylamine salts of alpha acids (DCHA-alpha acids) and beta acids (DCHA-beta acids), which are easier to use and more stable than ICE-3.