Abstract
The formation of total volatiles and yeast growth were inversely related to the CO2 counterpressure applied during 100-liter fermentations. However, the rate of fermentation was relatively unaffected. Thus, 8 psig (22.7 psia) CO2 counterpressure in a 22°C fermentation repressed both total yeast growth and total volatiles concentration to the levels present in a 15°C fermentation with 0 psig (14.7 psia) CO2 counterpressure. The repression of individual volatile compounds, however, was not uniform. The influence of temperature, agitation, and CO2 counterpressure upon the dissolved CO2 concentration during active fermentation was quantified. It was shown that the degree of supersaturation (termed the supersaturation coefficient), which is the ratio of the dissolved CO2 concentration during active fermentation to the CO2 saturation level, was constant over the ranges of temperature and CO2 counterpressure examined, at constant agitation. The supersaturation coefficient varied inversely and in a linear manner with agitation. At sufficiently high agitation, no CO2 supersaturation existed during active fermentation, thus the supersaturation coefficient was 1.0.