Determination of Isohumulone and Isocohumulone in Beer by HPLC¹

1 Presented at the 44th Annual Meeting, Toronto, Canada, May 1978.

Abstract

A high-pressure liquid chromatographic (HPLC) method is described for separating and measuring isohumulone and isocohumulone in beer. A trimethylpentane extract is dried and resuspended in methanol containing an internal standard. Paired-ion chromatography is achieved by adding to the mobile phase a large organic counter-ion that complexes with the oppositely charged ionic species, the iso-a-acids. The complexes are then chromatographed as non-ionic species on a reverse-phase column. Ion-exchange chromatography is used to calibrate the method. Wort, beer, and beer fractions were analyzed to demonstrate the method's application.

• Beer
• High-pressure liquid chromatography
• Iso-α-acids
• Isocohumulone
• Isohumulone