Abstract
In 1988, several Pediococcus strains that could not be visibly detected on culture media such as NBB, VLB-S7, and KL-2B within seven days' incubation, were isolated from various brewing environments. Consequently, KOT (Kirin-Ohkochi-Taguchi) medium was developed for the accurate detection of such beer spoilage lactic acid bacteria. KOT medium was found to support better growth of these sluggish lactic acid bacteria than other media studied.