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Original Articles

Morphological Changes Associated with the Cellular Aging of a Brewing Yeast Strain

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Pages 121-126 | Published online: 06 Feb 2018
 

Abstract

The yeast Saccharomyces cerevisiae possesses a finite replicative lifespan—each individual cell is capable of only a limited number of divisions before the onset of senescence and, finally, death. Methods to investigate the aging process in laboratory yeast strains have been developed but have never been applied to the less genetically tractable brewing yeasts. Therefore, the effects of cellular aging on fermentation performance are not known. Characterization of the aging profile of a lager yeast strain and age-related changes in cellular morphology are reported here. It is suggested that aging and senescence of yeast cells may play a role in “hung fermentations” and related problems sometimes experienced by the brewing industry.

Resumen

La levadura Saccharomyces cerevisiae posee un promedio de vida replicativo finito: cada célula individual es capaz solamente de un número limitado de divisiones antes del inicio de vejez y, finalmente, muere. Métodos para investigar el proceso de envejecimiento en cepas de levadura de laboratorio han sido desarrollados, pero nunca han sido aplicados en levaduras de proceso, menos manejables genéticamente. Por lo tanto los efectos del envejecimiento celular en el comportamiento de fermentación no son conocidos. Caracterización del perfil de envejecimiento de una cepa de levadura lager y cambios relacionados a la edad en la morfología celular son reportados aquí. Se sugiere que el envejecimiento y senectud de las células de levaduras pueden. jugar un papel en “fermentaciones lentas” y problemas similares aigunas veces experimentados por la industria cervecera.

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