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Abstract
Most of the isomerized and reduced hop extracts being used to bitter beer today, such as iso-α-acids, rho-iso-α-acids, tetrahydro-iso-α-acids, and hexahydro-iso-α-acids, are formulated and sold based on spectrophotometric analysis. Isomerized and reduced α-acids absorb ultraviolet and visible light, making quantitative analysis by spectrophotometric analysis a simple and accurate tool. Although these hop products have been in use for over 30 years, there have been no published procedures for measuring their concentration by spectrophotometric analysis. This article presents a simple and convenient spectrophotometric test method to measure the concentrations of isomerized and reduced α-acids in alkaline methanol.
RESUMEN
La mayoría de los extractos de lúpulo isomerizados y reducidos que se utilizan para amargar cerveza hoy, tal como iso-alfa-ácidos, rho-isoalpha-ácidos, tetrahydro-iso-alpha-ácidos, y hexahydro-iso-alpha-ácidos, se formulan y se venden a base de análisis espectrofotometrico. Los alpha-ácidos isomerizados y reducidos absorben luz ultravioleta y visible, haciendo del análisis espectrofotometrico una simple y exacta herramienta de análisis cuantitativo. Aunque estos productos se han utilizado por mas de 30 años, no se ha publicado un procedimiento para medir sus concentraciones a través de análisis espectrofotometrico. Este articulo presenta un método de prueba espectrofotometrica simple y conveniente para medir las concentraciones de alfa-ácidos isomerizados y reducidos en metanol alcalino.