Continual pH lowering and manganese dioxide solubilization in the rhizosphere of the Mn-hyperaccumulator plant Chengiopanax sciadophylloides

Abstract

The Japanese woody plant Chengiopanax sciadophylloides is well known for its extraordinary accumulation of manganese (Mn), and is used as a model for studying Mn uptake and utilization by plants. To clarify the role of manganese dioxide (MnO₂) solubilization for Mn acquisition and further Mn hyperaccumulation in this plant, we examined the lowering of pH in the rhizosphere and Mn accumulation of this plant using regenerated plants. Plants regenerated from C. sciadophylloides calli lowered the pH of the culture broth continuously and simultaneously solubilized MnO₂ added to the medium. The Mn content of the plant increased up to 1,300 mg kg⁻¹ within 4 weeks of culture. Release of protein or specific organic acid from the roots was not observed. The medium used for plant culture maintained MnO₂ solubilization ability after removal of the plant; however, this ability was lost by adjustment to the same medium pH of pre-culture conditions. In addition, pH lowering and MnO₂ solubilization were suppressed by adding 1 mmol L⁻¹ of the plasma H⁺-ATPase inhibitor Na₃VO₄ to the medium, and completely inhibited when 5 mmol L⁻¹ of Na₃VO₄ was added. These results suggested that H⁺ leaking from plasma H⁺-ATPase plays an important role in MnO₂ solubilization in the rhizosphere of C. sciadophylloides and in Mn accumulation in this plant.

Key words:

• Chengiopanax sciadophylloides
• H⁺-ATPase
• hyperaccumulator
• manganese
• pH lowering

ACKNOWLEDGMENTS

This work was supported in part by Grant-in-Aid for Young Researchers (B) to T. Mizuno (No. 181878004500) and Grant-in-Aid for Scientific Research on Priority Areas “Nutrient uptake and transport in plants—Identification of molecules responsible for transport and their regulation mechanisms” to H. Obata and T. Mizuno (No. 181805600900) from the Ministry of Education, Culture, Sports, Science and Technology of Japan. This work was also supported in part by Grant-in-Aid for Scientific Research (C) to H. Obata (No. 181551007300) from the Japan Society for the Promotion of Science.