Abstract
XRCC1 is an essential protein required for the maintenance of genomic stability through its implication in DNA repair. The main function of XRCC1 is associated with its role in the single-strand break (SSB) and base excision repair (BER) pathways that share several enzymatic steps. We show here that the polymorphic XRCC1 variant R194W presents a defect in its interaction with the DNA glycosylase OGG1 after oxidative stress. While proficient for single-strand break repair (SSBR), this variant does not colocalize with OGG1, reflecting a defect in its involvement in BER. Consistent with a role of XRCC1 in the coordination of the BER pathway, induction of oxidative base damage in XRCC1-deficient cells complemented with the R194W variant results in increased genetic instability as revealed by the accumulation of micronuclei. These data identify a specific molecular role for the XRCC1-OGG1 interaction in BER and provide a model for the effects of the R194W variant identified in molecular cancer epidemiology studies.
ACKNOWLEDGMENTS
We thank the members of our laboratories for critical reading, discussions, and suggestions. We also thank the IRCM microscopy and cytometry facilities, Françoise Hoffschir for help with micronucleus determination, and Heinrich Leonhardt (University of Munich) for kindly sending us the LIG3-RFP plasmid.
This work was funded by INSERM and grants from the Association pour la Recherche sur le Cancer (PJA 20131200165 to J.P.R.), the CEA Radiobiology program (to J.P.R. and A.C.), and the Deutsche Forschungsgemeinschaft (EP11/8-2 to B.E.). Exchanges between the laboratories of J.P.R. and B.E. were supported by the PROCOPE program (28345TK).