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Article

Regulatory Role of Human AP-Endonuclease (APE1/Ref-1) in YB-1-Mediated Activation of the Multidrug Resistance Gene MDR1

, , , , , , , & show all
Pages 7066-7080 | Received 13 Feb 2008, Accepted 08 Sep 2008, Published online: 27 Mar 2023
 

Abstract

Human AP-endonuclease (APE1/Ref-1), a central enzyme involved in the repair of oxidative base damage and DNA strand breaks, has a second activity as a transcriptional regulator that binds to several trans-acting factors. APE1 overexpression is often observed in tumor cells and confers resistance to various anticancer drugs; its downregulation sensitizes tumor cells to such agents. Because the involvement of APE1 in repairing the DNA damage induced by many of these drugs is unlikely, drug resistance may be linked to APE1's transcriptional regulatory function. Here, we show that APE1, preferably in the acetylated form, stably interacts with Y-box-binding protein 1 (YB-1) and enhances its binding to the Y-box element, leading to the activation of the multidrug resistance gene MDR1. The enhanced MDR1 level due to the ectopic expression of wild-type APE1 but not of its nonacetylable mutant underscores the importance of APE1's acetylation in its coactivator function. APE1 downregulation sensitizes MDR1-overexpressing tumor cells to cisplatin or doxorubicin, showing APE1's critical role in YB-1-mediated gene expression and, thus, drug resistance in tumor cells. A systematic increase in both APE1 and MDR1 expression was observed in non-small-cell lung cancer tissue samples. Thus, our study has established the novel role of the acetylation-mediated transcriptional regulatory function of APE1, making it a potential target for the drug sensitization of tumor cells.

SUPPLEMENTAL MATERIAL

Supplemental material for this article may be found at http://mcb.asm.org/ .

ACKNOWLEDGMENTS

We thank George W. Teebor, New York University School of Medicine, for the YB-1 expression plasmid; Michael Gottesman, NCI, for the MCF-7MDR1 cell line; and Lori Bernstein, Texas A&M University System Health Science Center, College Station, TX, for the FLAG-YB-1 construct. We thank Peter B. Ernst, University of Virginia, for statistical analysis. We also thank D. Konkel for critically reading and editing the manuscript and Wanda Smith for secretarial assistance.

This work was supported by American Heart Association grants 0565008Y (to K.K.B.), R01 ES08457, R01 CA53791, and P50 ES66076 (to S.M.).

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