Hepatocyte Nuclear Factor 4α, a Key Factor for Homeostasis, Cell Architecture, and Barrier Function of the Adult Intestinal Epithelium

Abstract

Hepatocyte nuclear factor 4α (HNF-4α) is a transcription factor which is highly expressed in the intestinal epithelium from duodenum to colon and from crypt to villus. The homeostasis of this constantly renewing epithelium relies on an integrated control of proliferation, differentiation, and apoptosis, as well as on the functional architecture of the epithelial cells. In order to determine the consequences of HNF-4α loss in the adult intestinal epithelium, we used a tamoxifen-inducible Cre-loxP system to inactivate the Hnf-4a gene. In the intestines of adult mice, loss of HNF-4α led to an increased proliferation in crypts and to an increased expression of several genes controlled by the Wnt/β-catenin system. This control of the Wnt/β-catenin signaling pathway by HNF-4α was confirmed in vitro. Cell lineage was affected, as indicated by an increased number of goblet cells and an impairment of enterocyte and enteroendocrine cell maturation. In the absence of HNF-4α, cell-cell junctions were destabilized and paracellular intestinal permeability increased. Our results showed that HNF-4α modulates Wnt/β-catenin signaling and controls intestinal epithelium homeostasis, cell function, and cell architecture. This study indicates that HNF-4α regulates the intestinal balance between proliferation and differentiation, and we hypothesize that it might act as a tumor suppressor.

ACKNOWLEDGMENTS

We thank N. Comuce, C. Lasne, and V. Chauffeton for animal care. Electron microscopy analysis was performed by using facilities of the Centre de Recherche des Cordeliers, UMRS 872. We thank M. Pontoglio for helpful scientific discussions and access to unpublished results, J. M. Lacorte and V. Carrière for their help during genotyping and RT-PCR experiments, respectively, and M. Rousset for critical reading of the manuscript.

This work was supported by INSERM, France, and Pierre & Marie Curie University, Paris, France. A.-L. Cattin is the recipient of a doctoral fellowship from the Ministère de l'Enseignement Supérieur et de la Recherche.