Abstract
Rett syndrome (RTT) is a severe neurological disorder caused by mutations in the X-linked MECP2 gene, which encodes a methyl-CpG binding transcriptional repressor. Using the Mecp2-null mouse (an animal model for RTT) and differential display, we found that mice with neurological symptoms overexpress the nuclear gene for ubiquinol-cytochrome c reductase core protein 1 (Uqcrc1). Chromatin immunoprecipitation demonstrated that MeCP2 interacts with the Uqcrc1 promoter. Uqcrc1 encodes a subunit of mitochondrial respiratory complex III, and isolated mitochondria from the Mecp2-null brain showed elevated respiration rates associated with respiratory complex III and an overall reduction in coupling. A causal link between Uqcrc1 gene overexpression and enhanced complex III activity was established in neuroblastoma cells. Our findings raise the possibility that mitochondrial dysfunction contributes to pathology of the Mecp2-null mouse and may contribute to the long-known resemblance between Rett syndrome and certain mitochondrial disorders.
We thank Jim Selfridge and Hannah Moore for assistance and advice, Bird laboratory members and Cathy Abbott for critical comments on the manuscript, and John Findlay for assistance in using the electron microscope.
This research was funded by the Wellcome Trust, the Rett Syndrome Research Foundation, and Jeans for Genes (Rett Syndrome United Kingdom). S.K. was a Darwin Trust Scholar and now holds an RSRF fellowship. A.P. was supported by a BBSRC studentship.