Repression of Beta Interferon Gene Expression in Virus-Infected Cells Is Correlated with a Poly(A) Tail Elongation

Abstract

Expression of beta interferon (IFN-β) is transiently induced when Namalwa B cells (Burkitt lymphoma cell line) are infected by Sendai virus. In this study, we found that an elongation of the IFN-β mRNA could be detected in virus-infected cells and that such a modification was not observed when the IFN-β transcript was induced by a nonviral agent, poly(I-C). Treatment of the cells with a transcriptional inhibitor (actinomycin D or 5,6-dichloro-1-D-ribofuranosylbenzimidazole) resulted in further elongation of the transcript. Characterization of the elongated IFN-β transcript by primer extension and RNase H treatment showed that the modification was a result of an elongated poly(A) tail of up to 400 nucleotides. We conclude that the poly(A) tail elongation of the IFN-β transcript is associated with the viral infection. Furthermore, the presence of the elongated IFN-β transcript correlated with a decrease of IFN-β protein in the medium and in cell extracts. Sucrose gradient analysis of cytoplasmic extracts showed that IFN-β transcripts with elongated poly(A) tails were found in the nonpolysomal fractions, whereas the shorter transcripts could be detected in both polysomal and nonpolysomal fractions. A longer form of the IFN-β mRNA was also found in the nonpolysomal fractions of cells not treated with transcriptional inhibitors. Thus, the observed regulation of IFN-β mRNA is not entirely dependent on the inhibition of transcription. To our knowledge, this study provides the first example of a poly(A) tail elongation in somatic cells that negatively influences gene expression in vivo.