Saturation Mutagenesis of 5S rRNA in Saccharomyces cerevisiae

Abstract

rRNAs are the central players in the reactions catalyzed by ribosomes, and the individual rRNAs are actively involved in different ribosome functions. Our previous demonstration that yeast 5S rRNA mutants (called mof9) can impact translational reading frame maintenance showed an unexpected function for this ubiquitous biomolecule. At the time, however, the highly repetitive nature of the genes encoding rRNAs precluded more detailed genetic and molecular analyses. A new genetic system allows all 5S rRNAs in the cell to be transcribed from a small, easily manipulated plasmid. The system is also amenable for the study of the other rRNAs, and provides an ideal genetic platform for detailed structural and functional studies. Saturation mutagenesis reveals regions of 5S rRNA that are required for cell viability, translational accuracy, and virus propagation. Unexpectedly, very few lethal alleles were identified, demonstrating the resilience of this molecule. Superimposition of genetic phenotypes on a physical map of 5S rRNA reveals the existence of phenotypic clusters of mutants, suggesting that specific regions of 5S rRNA are important for specific functions. Mapping these mutants onto the Haloarcula marismortui large subunit reveals that these clusters occur at important points of physical interaction between 5S rRNA and the different functional centers of the ribosome. Our analyses lead us to propose that one of the major functions of 5S rRNA may be to enhance translational fidelity by acting as a physical transducer of information between all of the different functional centers of the ribosome.

ACKNOWLEDGMENTS

We extend our warmest thanks to M. Nomura for his kind gift of the rdn1ΔΔ yeast strain and to A. Jacobson for the upf1Δ strain. We also thank Manan Patel and Deepu Abraham for technical help and Jason Harger, Kristi Muldoon, Ewan Plant, and Gary Brewer for critical reviews of the manuscript.

This work was supported by grants to J.D.D. from the National Institutes of Health (R01 GM58859 and R01 GM62143).