Abstract
Nuclear pore complexes (NPCs) traverse the nuclear envelope (NE), providing a channel through which nucleocytoplasmic transport occurs. Nup358/RanBP2, Nup214/CAN, and Nup88 are components of the cytoplasmic face of the NPC. Here we show that Nup88 localizes midway between Nup358 and Nup214 and physically interacts with them. RNA interference of either Nup88 or Nup214 in human cells caused a strong reduction of Nup358 at the NE. Nup88 and Nup214 showed an interdependence at the NPC and were not affected by the absence of Nup358. These data indicate that Nup88 and Nup214 mediate the attachment of Nup358 to the NPC. We show that localization of the export receptor CRM1 at the cytoplasmic face of the NE is Nup358 dependent and represents its empty state. Also, removal of Nup358 causes a distinct reduction in nuclear export signal-dependent nuclear export. We propose that Nup358 provides both a platform for rapid disassembly of CRM1 export complexes and a binding site for empty CRM1 recycling into the nucleus.
We thank Terry Allen and Sandra Rutherford for help with EM, Reuven Agami for sharing RNAi technology, Beric Henserson and Josean Rodriguez for plasmid reagents and advice, Volker Cordes, Frauke Melchior, Yoshiuki Kanai, and Tetsuo Kubota (University of Tokyo, Japan) for antibody reagents, Lauran Oomen and Lenny Brocks for valuable assistance in confocal microscopy, Christos Samakovlis for discussions and communicating unpublished results, and Frauke Melchior, Sebastian Nijman, Judith Boer, Dieuwke Engelsma, and Jolita Hendriksen for critically reading the manuscript.
R.B. is supported by a grant from The Netherlands Science Foundation Earth and Life Sciences. H.P. is supported by a Marie Curie European Community Training and Mobility Fellowship.