Abstract
We investigated the function of the histone H3/H4 chaperones anti-silencing function 1 (Asf1p) and chromatin assembly factor 1 (CAF-1) in global transcriptional regulation in budding yeast. Deletion of ASF1 or CAF-1 components led to global transcriptional misregulation, both activation and repression, of genes scattered throughout the 16 yeast chromosomes. To investigate direct effects on gene regulation, we developed an approach to destabilize Asf1p that results in its rapid degradation within minutes of transcriptional repression. Upon degradation of Asf1p, rapid global changes in gene expression occur without the requirement for passage through S phase or de novo protein synthesis. In particular, we demonstrate that the previously reported influence of Asf1p on histone gene expression is not a direct effect of loss of Asf1p. These data indicate that the histone chaperones CAF-1 and Asf1p regulate the gene expression of a broad array of genes in yeast and, in the case of Asf1p, this is likely to be due to a direct role in chromatin modulation during transcriptional regulation.
SUPPLEMENTAL MATERIAL
Supplemental material for this article may be found at http://mcb.asm.org/.
ACKNOWLEDGMENTS
We thank Melissa Adkins, Jeffrey Linger, Beth Tamburini, and Nicholas White for critical reading of the manuscript. We acknowledge David Bentley and Mark Longtine for plasmids and Lorraine Pillus for strains. We are highly grateful to Larry Hunter for advice with the statistical analyses of the microarray results and to the University of Colorado Cancer Center Affymetrix microarray and flow cytometry core facilities for assistance on this project.
J.K.T. is a Leukemia and Lymphoma Society Scholar. This study was supported by a grant from the National Institutes of Health (GM64475) to J.K.T. and supported in part by research grant FY02-201 from the March of Dimes Birth Defects Foundation.