ACE1 Transcription Factor Produced in Escherichia coli Binds Multiple Regions within Yeast Metallothionein Upstream Activation Sequences

Abstract

The ACE1 protein of Saccharomyces cerevisiae was expressed as a trpE-ACE1 fusion protein in Escherichia coli arid shown to bind CUP1 upstream activation sequences at multiple regions in a copper-inducible manner. These binding sites contain within them the sequence 5'-TC(T)_4-6GCTG-3', which we propose constitutes an important part of the ACE1 consensus recognition sequence.