Efficient Transcription of the Glycolytic Gene ADH1 and Three Translational Component Genes Requires the GCR1 Product, Which Can Act Through TUF/GRF/RAP Binding Sites

Abstract

Glycolytic gene expression in Saccharomyces cerevisiae is thought to be activated by the GCR and TUF proteins. We tested the hypothesis that GCR function is mediated by TUF/GRF/RAP binding sites (UAS_RPG elements). We found that UAS_RPG-dependent activation of a heterologous gene and transcription of ADH1, TEF1 TEF2, and RP59 were sensitive to GCR1 disruption. GCR is not required for TUF/GRF/RAP expression or in vitro DNA-binding activity.