Substitution of Foreign Protein Sequences into a Chimeric Toxin Composed of Transforming Growth Factor a and Pseudomonas Exotoxin

Waldemar Debinski1 Laboratory of Molecular Biology, Division of Cancer Biology and Diagnosis and Centers, National Cancer Institute, National Institutes of Health, Building 37, Room 4E16, 9000 Rockville Pike, Bethesda, Maryland20892

Clay B. Siegall1 Laboratory of Molecular Biology, Division of Cancer Biology and Diagnosis and Centers, National Cancer Institute, National Institutes of Health, Building 37, Room 4E16, 9000 Rockville Pike, Bethesda, Maryland20892

David Fitzgerald1 Laboratory of Molecular Biology, Division of Cancer Biology and Diagnosis and Centers, National Cancer Institute, National Institutes of Health, Building 37, Room 4E16, 9000 Rockville Pike, Bethesda, Maryland20892

Ira Pastan1 Laboratory of Molecular Biology, Division of Cancer Biology and Diagnosis and Centers, National Cancer Institute, National Institutes of Health, Building 37, Room 4E16, 9000 Rockville Pike, Bethesda, Maryland20892

Abstract

TGFα-PE40 is a chimeric toxin made by replacing domain Ia of Pseudomonas exotoxin (PE) with transforming growth factor a (TGFα). We have now replaced a portion of domain Ib of PE with different polypeptides or an extra domain III of PE in transforming growth factor a-PE40 and maintained cell killing. Thus, TGFα-PE40 can be used to transport foreign protein sequences into the cytosol of cells.

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Substitution of Foreign Protein Sequences into a Chimeric Toxin Composed of Transforming Growth Factor a and Pseudomonas Exotoxin

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Substitution of Foreign Protein Sequences into a Chimeric Toxin Composed of Transforming Growth Factor a and Pseudomonas Exotoxin

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