The TIS11 Primary Response Gene Is a Member of a Gene Family That Encodes Proteins with a Highly Conserved Sequence Containing an Unusual Cys-His Repeat

Brian C. Varnum1 Department of Biological Chemistry, Molecular Biology Institute, University of California Los Angeles Center for the Health Sciences, Los Angeles, California90024

Qiufu Ma1 Department of Biological Chemistry, Molecular Biology Institute, University of California Los Angeles Center for the Health Sciences, Los Angeles, California90024

Tianhuai Chi1 Department of Biological Chemistry, Molecular Biology Institute, University of California Los Angeles Center for the Health Sciences, Los Angeles, California90024

Bradley Fletcher1 Department of Biological Chemistry, Molecular Biology Institute, University of California Los Angeles Center for the Health Sciences, Los Angeles, California90024

Harvey R. Herschman1 Department of Biological Chemistry, Molecular Biology Institute, University of California Los Angeles Center for the Health Sciences, Los Angeles, California90024;2 Laboratory of Biomedical and Environmental Sciences, University of California Los Angeles Center for the Health Sciences, Los Angeles, California90024

Abstract

The TIS11 primary response gene is rapidly and transiently induced by both 12-O-tetradecanoylphorbol- 13-acetate and growth factors. The predicted TISH protein contains a 6-amino-acid repeat, YKTELC. We cloned two additional cDNAs, TISHb and TISHd, that contain the YKTELC sequence. TIS11, TIS11b, and TIS11d proteins share a 67-amino-acid region of sequence similarity that includes the YKTELC repeat and two cysteine-histidine containing repeats. TIS11 gene family members are not coordinately expressed: (i) unlike TIS11, the TIS11b and TIS11d mRNAs are detectable in quiescent Swiss 3T3 cells and are not dramatically induced by 12-O-tetradecanoylphorbol-13-acetate; (ii) cycloheximide superinduction does not occur for TIS11b and TIS11d; and (iii) unlike TIS11, TIS11b expression is extinguished in PC12 pheochromocytoma cells.

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The TIS11 Primary Response Gene Is a Member of a Gene Family That Encodes Proteins with a Highly Conserved Sequence Containing an Unusual Cys-His Repeat

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The TIS11 Primary Response Gene Is a Member of a Gene Family That Encodes Proteins with a Highly Conserved Sequence Containing an Unusual Cys-His Repeat

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