Transcriptional Repression of the E2-Containing Promoters EIIaE, c-myc, and RB1 by the Product of the RB1 Gene

Abstract

The protein product of the retinoblastoma susceptibility gene, p110^RB1, is a nuclear phosphoprotein [W. H. Lee, J.Y. Shew, F. D. Hong, T. W. Sery, L. A. Donoso, L. J. Young, R. Bookstein, and E. Y. Lee, Nature (London) 329:642-645, 1987] with properties of a cell cycle regulator (K. Buchkovich, L. A. Duffy, and E. Harlow, Cell 58:1097-1105, 1989; P. L. Chen, P. Scully, J. Y. Shew, J. Y. Wang, and W. H. Lee, Cell 58:1193-1198, 1989; J. A. DeCaprio, J. W. Ludlow, D. Lynch, Y. Furukawa, J. Griffin, H. Piwnica-Worms, C. M. Huang, and D. M. Livingston, Cell 58:1085-1095, 1989; and K. Mihara, X. R. Cao, A. Yen, S. Chandler, B. Driscoll, A. L. Murphree, A. TAng, and Y. K. Fung, Science 246:1300-1303, 1989). Although the mechanism of action of p110^RB1 remains unknown, several lines of evidence suggest that it plays a role in the regulation of transcription. We now show that overexpression of p110^RB1 causes repression of the adenovirus early promoter EHaE and the promoters of two cellular genes, c-myc and RB1, both of which contain E2F-binding motifs. Mutation of the E2 element in the c-myc promoter abolishes p110^RB1 repression. We also demonstrate that a p110^RB1 mutant, which is refractory to cell cycle phosphorylation but intact in E1a/large T antigen-binding properties, represses EIIaE with 50- to 80-fold greater efficiency than wild-type p110^RB1. These data provide evidence that hypophosphorylated p110^RB1 actively represses expression of genes with promoters containing the E2F-binding motif (E2 element).